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环保商城 Fascin1 Protein

Fascin1 Protein

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更新时间:2024-04-24

有效日期:还剩80

产品详情

Fascin1 Protein [10150]
[100 µg]


Catalog Number:  10150
Product Name:  Fascin1 Protein
Synonyms:  Fascin homolog 1, actin-bundling protein, FSCN1, SNL, p55
Source:   Human, recombinant full length, his-tag
Expression Host:   E. coli
Molecular Weight:   55 kDa
Purity:   >95% by SDS-PAGE
Introduction:   Fascin is an ~58 kDa monomeric actin filament bundling protein. It is required to maximally cross-link the actin filaments into straight, compact, and rigid bundles, and contributes to the formation of filopodia that are critical for cell migration. Elevated levels of fascin have been found in metastatic tumors and are correlated with clinically aggressive phenotypes, poor prognosis, and shorter survival.
Amino Acid Sequence   (1-493)
MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLG 
RYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSV 
HIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVA 
RPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNV 
STRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRD 
RRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYD 
VFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKA 
SAETVDPASLWEY
Properties
Physical Appearance (form):   Dissolved in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Physical Appearance (form):  White or clear.
Concentration:  1 mg/mL
Storage:   -80°C
Preparation Instructions:  
Centrifuge the vial before open the cap and reconstitute in water. Adding of 10 mM β-mercaptoethanol or 1 mM DTT into the solution to protect the protein is recommended and using of non-ionic detergents such as n-Dodecyl β-D-maltoside(DoDM) or polyethylene detergents (e.g. C12E10) also help to stabilize the protein. Avoid repeated freezing and thawing after reconstitution. The purity of His6-tagged. Fascin was determined by SDS-PAGE and Coomassie Brilliant Blue Staining
References:  
1. Mosialos, G. et al., Am. J. Path. 148: 593-600, 1996. 
2. Ono, S. et al., J. Biol. Chem. 272: 2527-2533, 1997. 
3. Pinkus, G. S. et al., Am. J. Path. 150: 543-562, 1997.
Datasheet:  



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发布询价单

武汉纽斯特生物科技有限公司

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乐亮明

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